该方法利用肝素钠干扰 Cas12a 与 crRNA（CRISPR RNA）的结合过程，精准调节 Cas12 的顺式切割能力，等温扩增产物的积累会产生明显荧光信号。此外，这种通用检测方法适用于经典和非最优的前间区序列邻近基序（PAM），以及多种 Cas12a 亚型，如 LbCas12a、AsCas12a 和 ...

该研究对用于 CRISPR 基因编辑治疗的 Cas 核酸酶 SaCas9 和 AsCas12a 进行了理性工程化改造，设计出了具有最小化免疫原性的 SaCas9 和 AsCas12a 突变体。

Get Instant Summarized Text (Gist) CRISPR nucleases, Cas9 and Cas12, have been engineered to evade immune detection, addressing a key challenge in CRISPR-based therapies. By identifying and ...

Researchers at the Broad Institute of MIT and Harvard and Cyrus Biotechnology have now engineered two CRISPR nucleases, Cas9 and Cas12, to mask them from the immune system. The team identified ...

By creating gene editors not found in nature, or optimizing existing editors, AI can improve the accuracy, effectiveness, and accessibility of gene editing.

Efficient and site-specific genome engineering can be achieved based on programmable nucleic acid cleavage using CRISPR-Cas surveillance complexes. Structure-function studies on single component Cas ...

CRISPR-Cas系统源自细菌防御病毒入侵的机制，其中用到的Cas蛋白质是由更小的分子演变而来，TnpB就是该技术常用蛋白质之一Cas12的“祖先”。 Cas蛋白 ...

CRISPR-based detection has long been challenged by the separation of amplification and detection steps. In this study, Cheng et al., show that heparin sodium modulates Cas12 cleavage activity and ...