近日，厦门大学生命科学学院刘亮教授团队在《Nucleic Acids Research》期刊上在线发表了题为“DNA target binding-induced pre-crRNA processing in type II and V ...

When the CRISPR Cas9 protein is added to a cell along with a piece of guide RNA, the Cas9 protein hooks up with the guide RNA and then moves along the strands of DNA until it finds and binds to a ...

可识别并切割非完全配对的双链DNA、十字形结构DNA、 Holliday交叉DNA、异源双链DNA，或缓慢地切割带有切刻的双链DNA；可有效识别大于1个碱基的基因错配，广泛应用于由CRISPR-Cas9、锌指核酸酶(ZFNs ...

The core components of CRISPR-based genome-editing therapies are bacterial proteins called nucleases that can stimulate ...

CRISPR-Cas9 genome editing exploits the CRISPR-Cas system to modify a genome in a targeted manner. Guided by RNA, the Cas9 endonuclease breaks DNA at a target sequence. Imprecise repair of the ...

CRISPR-Cas systems have been exploited for targeted genome editing. Applying the CRISPR-Cas9 system to edit high GC content genomes has been challenging due to its high off-target activity.

这组作者使用基因编辑工具CRISPR-Cas9，让NANOS2基因失去活性 ... 这组作者使用精原干细胞移植（SSCT）实现了睾丸结构正常的小鼠、猪和山羊产生 ...

CRISPR Therapeutics AG is a gene editing company, which engages in the development of transformative gene-based medicines for serious diseases using its proprietary CRISPR/Cas9 platform.

实验结果显示，target DNA确实可以在体外激活Cas12a的RuvC结构域来加工pre-crRNA的spacer区域。此外，团队成员也在其他V型CRISPR-Cas系统成员，如Cas12b、Cas12i和Cas12j，以及II型CRISPR-Cas9中观察到了这一现象。

Its CRISPR/Cas9 is a gene editing technology that allows for precise directed changes to genomic DNA. The company has a portfolio of therapeutic programs across a range of disease areas ...