A team of researchers from the Hefei Institutes of Physical Science of the Chinese Academy of Sciences has developed a ...

While single-cell CRISPR screens based on the WTA approach were limited to a few hundred perturbations, the targeted sequencing workflow presented here will allow genome-scale CRISPR screens.

CRISPR is a technology that can be used to edit genes and, as such, will likely change the world. The essence of CRISPR is simple: it’s a way of finding a specific bit of DNA inside a cell.

View Full Profile. Learn about our Editorial Policies. Like the human immune system, bacteria learn from past infections. CRISPR sequences—short snippets of DNA from previous viruses—guide destructive ...

The second tool is the CRISPR-Cas9 system, which allows easy and precise editing of any region of the genome. When it comes to traditional immortalized cell lines, such as HeLa or HEK293, cutting with ...

CRISPR-Cas9 genome editing exploits the CRISPR-Cas system to modify a genome in a targeted manner. Guided by RNA, the Cas9 endonuclease breaks DNA at a target sequence. Imprecise repair of the ...

To streamline and accelerate tool development, TMO has also integrated Gibson Assembly into its CRISPR workflows, making it easier to clone large DNA fragments efficiently. On December 20 ...

We also plan to expand our custom oligo offerings to support gene assembly, antibody screening, and CRISPR HDR donor workflows.” Bill Efcavitch, Co-Founder of Molecular Assemblies, said, “We are ...

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