Of paramount importance is the cell line. The cell culture media is the second factor, and third, the configuration of the bioreactor, including settings such as dissolved oxygen or agitation rate.

A PAT roadmap should address data analysis, aggregation, visualization, and the smart utility of those data for greater process understanding.

In contrast, single-cell Raman spectroscopy has emerged as a promising ... of microorganisms and offers advantages such as speed, culture-free operation, and multi-target detection.

The technology, FISH-scRACS-seq (Fluorescence In Situ Hybridization-guided Single-Cell Raman-activated Sorting ... conducted with complex soil and mixed-culture systems, FISH-scRACS-seq ...