The most straightforward way to examine the pattern of Cre-mediated recombination in the various tissues of the mouse is Southern blot analysis distinguishing wild-type, loxP-flanked and ...

To generate a tissue specific knockout mouse, researchers breed a mouse bearing a Cre transgene under a tissue- or cell-type specific or inducible promoter (A) with a homozygous floxed mouse (B). The ...

Related Links: Jackson Laboratory / Mutant Mouse Regional Resource Center (MMRRC ... On binding tamoxifen, the complex is translocated to the nucleus where cre activates recombination of loxP-flanked ...

The Yong Xu lab is interested in novel neural circuits, neurotransmitters and intra-neuronal signals that are critical for coordinated control of metabolism and behavior. We generate unique mouse ...

All our multiexpression systems (currently MultiBac, MultiMam and MultiColi) rely on in vitro Cre-loxP recombination of Acceptor and Donor plasmids to assemble multiprotein expression vectors. A ...

The cerebella from c-kit IRES-Cre mice injected with Cre-reporter virus at PND (pups shortly after birth) 0 or PND 64. Tissue was prepared from animals 27 and 14 days after injection ...