To site-specifically label proteins in living cells, researchers mutate an enzyme to recognize a small fluorophore and catalyze its attachment to a small peptide tag. Fluorescent protein fusions ...

Uniform protein labeling in intact tissues is a challenge. An approach that allows antibodies to seep within organs could ...

A strategy for labeling cell-surface proteins in living cells with small-molecule fluorophores allowed scientists to study receptor trafficking by single-cell FRET imaging in real time. When ...

However, this method has faced technical difficulties: Typically, the fluorescent component could only be attached to one protein at a time, and to label multiple proteins, cells usually had to be ...

A new technology developed at MIT enables scientists to label proteins across millions of individual cells in fully intact 3D ...

Imaging live cells with fluorescent proteins has long been a crucial technique for understanding cellular behavior. While ...

The CuRVE framework and its implementation in eFLASH mark a significant advancement in volumetric single-cell analysis.

Imaging live cells with fluorescent proteins has long been a crucial technique for understanding cellular behavior. While ...

This obstacle has now been cleared by the Stefan Kubicek-led research team at CeMM: Five different fluorescent colors can be ...

This darkened protein can be switched back to its original emission state by irradiation for several minutes at 405 nm. Fluorescent protein-labeling techniques previously were irreversible, precluding ...

In some areas of mouse brains, they found that two-thirds of the neurons expressing PV (a protein prominent in certain inhibitory neurons) according to antibody labeling, did not show any ...

Proteins are naturally fluorescent in the ultraviolet, offering an appealing approach to probe proteins in their native state without introducing any external fluorescent label. The UV ...