We assessed six cell lines known to harbor Nras gene mutations using our multicolor multiplex assay. Two of the cell lines, MOLT-4 and PA-1, contain mutations in codon 12, while the remaining four ...

Real-time PCR is the favored method for measuring gene ... To help researchers configure single- or multiplex assays, the Promega website will have a Plexor Primer Design tool that will recommend ...

PCR-Machines are based on Polymerase Chain Reaction (PCR) and produce copies of genes exponentially. The PCR cycling reactions are carried out in an automated thermocycler, which is capable of ...

How it Works: Real Time PCR Brendan Maher The instrumentation is basic: a thermal cycler for amplification, a light source for excitation of fluorescent probes (see chemistries below), a camera for ...

real-time PCR (qPCR), digital PCR (dPCR), reverse transcription PCR (RT-PCR), hot-start PCR, multiplex PCR, and other PCR techniques. The qPCR segment holds the largest share of the market.

Real-time PCR detects and quantifies DNA as the reaction progresses. This quantifies the specific amount of DNA present in a sample. The procedure requires specialized thermal cyclers with ...

Because RSV, influenza, and COVID-19 share symptoms, accurate testing is needed to distinguish one from the other. This CE-IVD marked multiplex PCR test is specifically designed to detect and ...

Continued innovation and investment in molecular diagnostics will ensure broader access and better preparedness for HMPV, its variants and other respiratory illnesses.