PCR amplifies DNA in a three-step process: denaturation, which melts double-stranded DNA into single strands; annealing, which lets small pieces of primer DNA bind to either side of the region of ...

PCR involves the enzymatic amplification of nucleic acid templates, and can be divided into four major steps, listed below. 1 Denaturation: Double-stranded DNA separates into single strands. Annealing ...

Note, the pH is 9.2 (the optimal for long PCR) without adjustment ... 3| Add 5.5 μl of genomic DNA at 10 ng/μl to the master mix (Step 1) and mix thoroughly. 4| Distribute 48 μl to each ...

PCR, or Polymerase Chain Reaction, is a revolutionary molecular biology technique that allows for the rapid amplification of specific DNA sequences. It is an essential tool in various fields, ...