Real-time PCR is the favored method for measuring ... synthetic primers using the target DNA or cDNA as a template during repeated cycles of heat denaturation, primer annealing and primer extension.

The 10-fold improvement in detection sensitivity of these ET primers enables shorter PCR cycles and good results even with minute amounts of template DNA, a feature especially useful in forensic ...

Increasing the number of amplification cycles to 34 instead of 28 helps increase sensitivity ... Several components are involved in a PCR including template DNA, forward and reverse primers, DNA ...