PCR involves the enzymatic amplification of nucleic acid templates, and can be divided into four major steps, listed below. 1 Denaturation: Double-stranded DNA separates into single strands. Annealing ...

Then, to perform PCR, the DNA template that contains the target is added to a tube that contains primers, free nucleotides, and an enzyme called DNA polymerase, and the mixture is placed in a PCR ...

then we generally use 3–30 ng of template DNA per PCR (1,000–10,000 haploid genome equivalents). All steps are performed at room temperature (18–25 °C) except where indicated otherwise.