These data indicate that the small RNA molecules in the complex function only as a sequence-specific tag to deposit the machinery on the mRNA. It then becomes important to characterize the ...

Specifically, one strand of a double-stranded siRNA molecule can be incorporated into a complex called RISC. This RNA-containing complex can then inhibit transcription of an mRNA molecule that has ...

The RISC, guided by the miRNA, binds to complementary sequences in the 3' untranslated region (UTR) of target messenger RNAs (mRNAs). This binding leads to translational repression or mRNA degradation ...

Fire and Mello were awarded the Nobel Prize in Physiology or Medicine in 2006 for their work on RNAi. siRNAs are 21-25 nucleotide dsRNA molecules that are perfectly complementary to their target mRNA.

RNA interference is a conserved biological response to double-stranded RNA that regulates gene expression. The response is mediated by small interfering RNAs (siRNAs), which guide the ...