Often when inoculating a BAP to observe hemoloysis patterns, investigators will also stab several times through the agar using an inoculating loop. This stab allows for the detection of streptolysin O ...

The remaining cultures (Streptococcus pneumoniae, and Streptococcus pyogenes) will be taken from the blood agar plates inoculated in the previous lab ... If the tube is colorless (picture on the right ...