Activation of caspase 3 and PARP cleavage, by western blot and immunohistochemistry, and DNA fragmentation, by gel electrophoresis and TUNEL, are frequently used as apoptosis assays in vivo.

Oxidative DNA injury is associated with commitment to apoptosis as indicated by caspase-3 expression and TUNEL positivity. The highly significant association between 8-OHdG and caspase-3 ...