To carry out real-time PCR, researchers have to choose not only what primers to design (see Box 2) but also what detection chemistry to use. In many cases these decisions will be influenced ...

The key to an optimal PCR experiment is primer design. In general, primers should be between 18 and 24 nucleotides long and have a GC content between 40 and 60 percent. Ideally primers should contain ...

PCR involves the enzymatic amplification of nucleic acid templates, and can be divided into four major steps, listed below. 1 Denaturation: Double-stranded DNA separates into single strands. Annealing ...

Most genotyping is performed by polymerase chain reaction (PCR) with defined oligonucleotide primers. In order to analyze the length of the PCR products by electrophoresis and a laser detection ...

PCR amplifies DNA in a three-step process: denaturation, which melts double-stranded DNA into single strands; annealing, which lets small pieces of primer DNA bind to either side of the region of ...