CombiningTvDAO-based surrogate selection marker withthe CRISPR/Cas9 system enables the selection ofCas9-free and multigene CRISPR mutants. Disclaimer: AAAS and EurekAlert! are not responsible for ...

此外，团队成员也在其他V型CRISPR-Cas系统成员，如Cas12b、Cas12i和Cas12j，以及II型CRISPR-Cas9中观察到了这一现象。与Cas12a稍不同，Cas9仅在单链靶标DNA存在的情况下才会启动对pre-crRNA的spacer区的反式切割，且Cas9的HNH和RuvC结构域对于pre-crRNA的反式切割都是必需的。

实现更特异、更高效的基因敲入（knockin）！ CRISPR/Cas9和其它基因编辑工具已经被成功应用于获得基因敲除突变（knockout），但众多事实证明在基因敲入上的应用有很大的难度。基因敲入所面临的主要难题是修复模板的制备和如何与Cas9-sgRNA核糖核蛋白复合物共导入 ...

CRISPR screening libraries are collections of carefully designed single-guide RNAs (sgRNAs) that allow researchers to ...

The new Express License service removes barriers to accessing this transformative gene editing technology for startups.An ...

Cas9, the first Cas protein repurposed for cutting DNA, introduces double-strand breaks that recruit DNA repair machinery. Despite its successes, the CRISPR-Cas9 system has several limitations, ...

"Our research interest is to identify these genes in order to better treat diseases." CRISPR screening methods can be used to systematically examine genes for their function in cells. "CRISPR is ...

When the CRISPR Cas9 protein is added to a cell along with a piece of guide RNA, the Cas9 protein hooks up with the guide RNA and then moves along the strands of DNA until it finds and binds to a ...

CRISPR Therapeutics AG is a gene editing company, which engages in the development of transformative gene-based medicines for serious diseases using its proprietary CRISPR/Cas9 platform.

Its CRISPR/Cas9 is a gene editing technology that allows for precise directed changes to genomic DNA. The company has a portfolio of therapeutic programs across a range of disease areas ...