With vasa-Cas9, high efficiency and self-limiting suppression was achieved. Applying the CRISPR-Cas9 system to edit high GC content genomes has been challenging due to its high off-target activity.
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Applying the CRISPR-Cas9 system to edit high GC content genomes has been challenging due to its high off-target activity. In this study, the authors addressed this issue by adding polyaspartate ...