迄今为止，大多数基于 CRISPR 的基因编辑疗法依赖于以下三种 Cas 核酸酶之一：化脓链球菌 Cas9 （SpCas9）、金黄色葡萄球菌 Cas9 （SaCas9）和氨基酸球菌 ...

实现更特异、更高效的基因敲入（knockin）！ CRISPR/Cas9和其它基因编辑工具已经被成功应用于获得基因敲除突变（knockout），但众多事实证明在基因敲入上的应用有很大的难度。基因敲入所面临的主要难题是修复模板的制备和如何与Cas9-sgRNA核糖核蛋白复合物共导入 ...

CombiningTvDAO-based surrogate selection marker withthe CRISPR/Cas9 system enables the selection ofCas9-free and multigene CRISPR mutants. Disclaimer: AAAS and EurekAlert! are not responsible for ...

"Our research interest is to identify these genes in order to better treat diseases." CRISPR screening methods can be used to systematically examine genes for their function in cells. "CRISPR is ...

Cas9, the first Cas protein repurposed for cutting DNA, introduces double-strand breaks that recruit DNA repair machinery. Despite its successes, the CRISPR-Cas9 system has several limitations, ...

Australian researchers have successfully introduced an improved version of Cas12a gene-editing enzyme in mice.

When the CRISPR Cas9 protein is added to a cell along with a piece of guide RNA, the Cas9 protein hooks up with the guide RNA and then moves along the strands of DNA until it finds and binds to a ...

In addition, some companies are using CRISPR for screening (which shouldn't be confused with genetic testing). With CRISPR screening, genes are edited in a way that makes them inoperative.

Australian cancer researchers are the first to establish a next-generation gene-editing tool for modeling and interrogating human disease.

CRISPR Therapeutics AG is a gene editing company, which engages in the development of transformative gene-based medicines for serious diseases using its proprietary CRISPR/Cas9 platform.