Semen smears were prepared by spreading the sperm suspension on a microscope the slide for subsequent sperm immunofluorescence staining. Semen samples were centrifuged at 800 ... samples were washed ...

Tissues were fixed by perfusion in 4% paraformaldehyde in PBS, pH 7.3. Tissues were removed and immersed in 2% osmium tetroxide in 0.1 M sodium phosphate, pH 7.4, to stain neutral lipids.

Countries with commitments under the Kyoto Protocol to limit or reduce greenhouse gas emissions must meet their targets primarily through national measures. As an additional means of meeting these ...

For immunoperoxidase staining, retinal sections were incubated ... retinal sections were postfixed in 1% osmium tetroxide in 0.1 M cacodylate buffer for 1 hour. After washing in cacodylate buffer ...

Collective migration plays critical roles in developmental, physiological and pathological processes, and requires a dynamic actomyosin network for cell shape change, cell adhesion and cell-cell ...