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After bead incubation with detection antibodies, 25 µL of streptavidin–PE was added for 20 min. After washing three times, the magnetic beads were enumerated using the Luminex 200 (Millipore, ...

Cy3是一种高亮度且光稳定性良好的红色荧光染料，而生物素则是一种能够与链霉亲和素（Streptavidin）或抗生物素蛋白（Avidin）高亲和力结合的分子。