Thereafter, 1:600 diluted Phalloidin–Tetramethylrhodamine B isothiocyanate (TRITC; Sigma-Aldrich, Ireland) in 1% BSA in PBS was applied for 20 min to stain the cell cytoskeleton followed by 4′, ...

Images were captured through ×100 oil objectives (NA 1.4) using tetramethylrhodamine (TRITC) and fluorescein isothiocyanate (FITC) filter sets and LeicaLAS microscope imaging software. The entire ...

Washed cells were next permeabilized with 0.05% saponin (84510, Sigma Aldrich) in 3% BSA-containing DPBS for 15 min at RT and stained with phalloidin-TRITC (P1951, Sigma Aldrich) and DAPI (D9542, ...

A screen for proteins that interact with β2-syntrophin led to the isolation of MAST205 (microtubule-associated serine/threonine kinase-205 kD) and a newly identified homologue, SAST (syntrophin ...

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On the seventh postoperative day, with n=8 animals per group, gavage was performed with dextran-TRITC 40 kDa (100 µL of 20 mg/mL solution diluted in PBS, Sigma-Aldrich, USA) in each animal. After 45 ...