With the dye, cells may be analyzed by fluorescence microscopy, flow cytometry and laser-scanning cytometry. The ability to identify cell cycle position through DNA content analysis is fundamental ...

Flow cytometry may be used to analyse cell cycle in live eGFP Gli2 transduced human keratinocytes, NTERTs cells by loading cells with Hoechst 33342 at 10 ug/ml for 45 mins at 37C. The determination of ...

The Flow Cytometry and Cell Separation Facility provides advanced cell sorting and cell analysis techniques including immunofluorescence detection of 18 colors, cell-cycle distribution, apoptosis ...

Moreover, each step in the flow of information from DNA to ... Often, this breakdown is linked to specific events in the cell. The eukaryotic cell cycle provides a good example of how protein ...

Incubate the cells with fixation buffer for 15 to 30 min at 4°C. (Cell aggregation can be avoided by vortexing prior to the addition of the fixation buffer.) Fixed cells should be washed and suspended ...