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Nature
23 小时
Detection of protein-protein interactions using the GST fusion protein pull-down technique
1| Incubate the cell lysate with 50 μl of a 50% slurry of glutathione-agarose beads and 25 μg of GST for 2 h at 4 °C with end-over-end mixing. The amount of lysate needed to detect an ...
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