N-terminally FLAG-tagged human NFIL3-T2A-GFP (WT or carrying the M170I mutation) was expressed transiently from a plasmid in HEK293T. The expression was driven by chicken actin promoter with the CMV ...

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A method for genetically barcoding Drosophila is developed and used to tag defined cell populations in vivo for single-cell transcriptomics experiments and to enable multiplexed behavioral analysis.

and 16.67 ng/well pMAX-GFP plasmid. With these methods, cells were typically transfected at approximately 90–95% efficiency. We tested various LgBiT:SmBiT molar transfection ratios with the construct ...

Catalog no. 18324012) was used for the transfection of TRPM3 into the cells in a 35 mm culture dish according to the manufacturer’s instructions. Successfully transfected cells were identified by ...

It is hard to picture a holiday feast without turkey. Whether thinking about Thanksgiving or Christmas, turkey is a healthful and lean source of protein that people can consume year-round ...

People with diabetes should eat as much protein as people who don't have diabetes. In a healthy diet, roughly 10% to 35% of your daily calories should come from protein. Protein itself does not have ...

Oregon House Bill 2531, the final stage of which took effect Jan. 1, has changed household lighting in the state. "Fluorescent lamps are now an item of the past," KPIC reported. The bill bans stores ...

Best whey-protein powders Best collagen-protein powder Best plant-based protein powders All the other protein powders I’ve tried Protein powders can be found in thousands of formulations on tens ...

Before choosing the best protein powders, our testers and dietitians evaluated more than 100 different animal-based and whey protein powders, considering factors like ingredients, taste, and value ...

(G) Wild-type BMDM cells were primed overnight with 1 μg/ml Pam3CSK4, followed by transfection of 2 μg/ml LPS using DOTAP with or without the incubation of VgrG2b wild-type (KI) or mutant (D883A) ...