From being a promising yet underexplored approach in the 1990s, mRNA therapeutics have evolved dramatically. The discovery of ...

This study assesses the effectiveness of qPCR in detecting PSA coding KLK3 mRNA for identifying lymph node metastases post-prostatectomy and explores the correlation between PSA-mRNA and biochemical ...

She is passionate about making complex scientific concepts accessible to readers. View Full Profile. Learn about our Editorial Policies. Quantitative polymerase chain reaction (qPCR) is a molecular ...

Until this past January, few outside academic and diagnostic labs knew what quantitative reverse-transcription PCR (RT-qPCR) was, much less its role in viral and bacterial disease detection.

"For large-scale mRNA production, I have always favored post-transcriptional enzymatic capping ... Explore how the design and application of appropriate assay controls ensure accurate and reproducible ...

Introduction MicroRNA (miRNA) molecules are a class of small non-coding RNA molecules (∼22 nucleotides), which target the 3’ untranslated region (3’ UTR) of mRNA and either induce mRNA degradation or ...

The effect of STF31 was determined in vitro by qPCR (mRNA expression of catabolic [Mmp3, Mmp13] and anabolic [Col2a1, Acan] markers), and ex vivo by histology (Safranin O staining) and ...

Effects of HDAC5 modulation on RA FLS gene expression were analyzed by custom qPCR array. Results Positive correlations were observed between ... DAS28: R=-0.567, P=0.011). HDAC5 mRNA expression was ...