Bradford protein assay - Wikipedia
The Bradford protein assay (also known as the Coomassie protein assay) was developed by Marion M. Bradford in 1976. [1] It is a quick and accurate [ 2 ] spectroscopic analytical procedure used to measure the concentration of protein in a solution.
Bradford Protein Assay - an overview | ScienceDirect Topics
First, the Bradford protein assay is much easier to use. It requires one reagent and 5 minutes to perform as compared to the three reagents and 30–40 minutes typical for the Lowry assay.
Bradford Assay | Thermo Fisher Scientific - US
Bradford assays are dye-binding assays for fast and simple protein quantification. The assay is performed at room temperature and no special equipment is required.
Bradford Protein Assay - Bio-Rad
The Bradford protein assay is a time-tested colorimetric assay. When the Bradford reagent (acidified Coomassie Brilliant Blue G-250) binds to proteins, the dye undergoes a color change in the visible spectrum, with the absorbance maximum moving from 470 to 595 nm.
[Abstract] The Bradford protein assay is used to measure the concentration of total protein in a sample. The principle of this assay is that the binding of protein molecules to Coomassie dye under acidic conditions results in a color change from brown to blue.
The Bradford assay is faster, involves fewer mixing steps, does not require heating, and gives a more stable colorimetric response, its response is prone to influence from non protein sources, particularly detergents, and becomes progressively more nonlinear at the
The Bradford Method For Protein Quantitation | SpringerLink
An assay originally described by Bradford (1) has become the preferred method for quantifying protein in many laboratories. This... A rapid and accurate method for the estimation of protein concentration is essential in various areas of biology and biochemistry.