2024年10月18日 · Confocal Laser Scanning Microscopy (CLSM) is one of a series of methods to generate slices from microscopic samples by means of optics. The sample stays intact, and the slicing may be repeated many times. True Confocal Scanning (TCS) is a technique, where only a single, diffraction limited spot is illuminated and observed at a time.

Personal Confocal Microscope. STELLARIS is a true confocal point scanning system with beam splitters and has Spectral Detection, Photon Counting Detectors, and Confocal Super Resolution! STELLARIS confocal microscope also offers the following modalities, including Autonomous Microscopy, Digital Light Sheet, Dynamic Signal Enhancement, and more.

In microscopy, our mission is to empower you to drive progress in science. To help you get closer to the truth hidden in every sample, we have completely re-imagined confocal microscopy. Our family of confocal microscopes – STELLARIS 5 and STELLARIS 8 …

2023年9月11日 · This article outlines the most important sensors used in confocal microscopy. By confocal microscopy, we mean "True Confocal Scanning", i.e. the technique that illuminates and measures one single point only. The aim is not to impart in-depth specialist knowledge, but to give the user a small but clear overview of the differences between the various technologies and to …

2022年7月21日 · With fluorescence lifetime imaging microscopy (FLIM) uncover a new dimension of life-science specimens and reveal insights not seen with conventional microscopy. The FLIM method Fluorescence lifetime imaging microscopy ( FLIM ) exploits the inherent properties of fluorescent dyes to produce an image.

2013年5月17日 · Fluorescence Recovery After Photobleaching (FRAP) has been considered the most widely applied method for observing translational diffusion processes of macromolecules. The resulting information can be used to determine kinetic properties, like the diffusion coefficient, mobile fraction, and transport rate of the fluorescently labeled molecules. FRAP employs …

2012年6月26日 · The perfect light source for confocal microscopes in biomedical applications has sufficient intensity, tunable color and is pulsed for use in lifetime fluorescence. Furthermore, it should offer means to avoid reflection of excitation light, and the coupling into the beam path must be efficient and homogeneous throughout the full visible spectrum. Such a source has been …

2023年12月6日 · Confocal microscopy is typically used to study subcellular dynamic events. Multiphoton microscopy allows excitation with longer wavelength light reducing photobleaching and extending cell viability. Finally, fluorescence lifetime imaging ( FLIM ) can be applied to study fast dynamic signaling events in cells.

2024年9月24日 · Immunofluorescence (IF) is a powerful method for visualizing intracellular processes, conditions and structures. IF preparations can be analyzed by various microscopy techniques (e.g. CLSM, Epifluorescence, TIRF, GSDIM), depending on the application or the researcher’s interest. Meanwhile, IF has become indispensable for a large number of research …

2024年5月7日 · The confocal principle is seamlessly integrated into standard-type light microscopes (although the technical integration requires quite a bit of modification). Unlike ordinary light microscopy, true confocal imaging demands …