Cross-linking is widely used to improve the water sensitivity of protein films. Cross-linking is defined as the development of a 3D network by binding the polymer chains together through physical, chemical, and enzymatic methods (intermolecular covalent interaction) (Zhang et al., 2022). Protein cross-linking depends on the amino acid composition and their reactivity, the …

The cross-linking degree of S8 is comparable to that of S6. The unique triple-helix structure of gelatin facilitates its flexibility and allows TGase to catalyze the formation of cross-links between gelatin molecules, leading to a higher degree of cross-linking even …

Sulfur Cross-linking Although relative amounts of crosslinking are estimated by physical properties, a rough estimate of the degree of curing is obtained by quantitating free sulfur (ISO 7269), extractable low relative mass polymeric species, or by determining the curing isotherm using differential scanning calorimetry.

However, if the degree of cross-linking is increased the distance between cross-links decreases and a tighter, less flexible network will be formed. Segmental motion will become more restricted as the degree of cross-linking increases so that the transition temperature will eventually reach the decomposition temperature.

2022年1月6日 · Summary Crosslinking mass spectrometry (crosslinking-MS) is a versatile tool providing structural insights into protein conformation and protein-protein interactions. Its medium-resolution residue-residue distance restraints have been used to validate protein structures proposed by other methods and have helped derive models of protein complexes by …

We investigated the ionic cross-linking mechanism of these hydrogels and compared their microstructure and mechanical properties. Our results highlight significant differences between GIM and CCIM hydrogels, with the CCIM method producing a …

2022年1月1日 · The formed ionically cross-linked chitosan hydrogels have shown low mechanical strength and release controlling capacity of encapsulated substances due to hydrophilicity and high solubility in the acidic environment. Therefore the variation of cross-linking, which is facilitated by its hydroxyl and amino groups, is very much essential [71].

2024年12月15日 · In this study, a double cross-linking strategy was employed using poly (vinyl alcohol) (PVA) and 3-aminopropyltriethoxysilane (KH550) to reinforce cellulose aerogels, forming a stable 3D network through hydrogen and chemical bonding.

Glutaraldehyde (GA) has seen extensive use as a cross-linking agent for biomedical applications such as enzyme and cell immobilization as well as hydrogel synthesis [115,117,118,119,120,230]. GA is a colorless liquid with a pungent odor at room temperature. It is a dialdehyde whose aldehydic groups are highly reactive and can form covalent bonds with functional groups such …

In light of the new model invoking participation of NEIL3 in cross-link repair, we consider the possibility that various BER glycosylases or other DNA-processing enzymes might participate in the unhooking of chemically diverse interstrand DNA cross-links.