EGFP is a basic (constitutively fluorescent) green fluorescent protein published in 1996, derived from Aequorea victoria. It is reported to be a rapidly-maturing weak dimer with moderate acid sensitivity.

SnapGene Viewer is free software that allows molecular biologists to create, browse, and share richly annotated sequence files. The maps, notes, and annotations in the zip file on this page …

Enhanced Green Fluorescent Protein (EGFP) is a genetically modified version of the Green Fluorescent Protein (GFP) that emits 35 times more fluorescence when excited with ultraviolet or blue light, making it much brighter.

For example, green fluorescent protein (GFP) has evolved from a little known protein to a common widely used tool in molecular biology and cell biology. It started with different spectral shifted variants of Aequorea victoria green fluorescent protein (GFP), including an enhanced GFP (eGFP) [59–65]. These GFP variants are particularly useful ...

Enhanced Green Fluorescent Protein (EGFP) is one of the most widely used engineered variants of the original wild-type Green Fluorescent Protein. Here, we report the high resolution (1.35 Å) structure of EGFP crystallised in its untagged sequence ...

2018年6月12日 · The main difference between GFP and EGFP is that the GFP (stands for Green Fluorescent Protein) is a protein that exhibits bright green fluorescence when exposed to blue light whereas the EGFP (stands for Enhanced Green Fluorescence Protein) exhibits stronger fluorescence than GFP.

From these experiments EGFP emerges as an ideal molecule for quantitative studies of intra and intercellular tagged-protein dynamics and fluorescence-activated cell sorting, but not for monitoring single-protein trafficking over extended periods of time.

2022年11月14日 · EGFP. Definition. GFP is a wild-type green fluorescent protein that is used in molecular cloning of non-mammalian cells EGFP is an enhanced form of GFP that can be used on mammalian cells during molecular cloning Origin. Wild-type, isolated from jellyfish (Aequorea victoria) Synthesized in the laboratory by creating enhanced mutants ...

2010年12月2日 · Enhanced Green Fluorescent Protein (EGFP) is a variant of wild-type Green Fluorescent Protein from the jellyfish Aequorea victoria, whose mutations S65T and F64L increase brightness and folding efficiency.

Enhanced green fluorescent protein (EGFP) is a widely used variant of GFP, which has mutations at two positions: F64L and S65T [9, 10]. EGFP is brighter and matures rapidly at 37°C than wild-type GFP [1, 9]. Protein engineering of EGFP has yielded several green variants with improved characteristics such as Emerald.