The RNA-induced silencing complex, or RISC, is a multiprotein complex, specifically a ribonucleoprotein, which functions in gene silencing via a variety of pathways at the transcriptional and translational levels. [1]

RNA诱导沉默复合体（RNA-induced silencing complex，RISC）：一种由 siRNA 与Argonaute蛋白和 Dicer酶 复合形成的复合物。 在RNAi中，利用siRNA的 反义链 切割靶 mRNA ，达到 基因沉默 。

RNA诱导沉默复合物(RNA-induced silencing complex,RISC),是 RNA干扰技术 中起作用的重要物质。 一定数量的外源性 双链RNA （dsRNA）进入细胞后，被类似于 核糖核酸酶 Ⅲ的Dicer 酶切割成短的21~23bp的双链 小干扰RNA （si RNA），si RNA与解旋酶和其它因子结合，形成RNA诱导 …

RNA interference is a powerful mechanism of gene silencing that underlies many aspects of eukaryotic biology. On the molecular level, RNA interference is mediated by a family of ribonucleoprotein complexes called RNA-induced silencing complexes (RISCs), which can be programmed to target virtually any nucleic acid sequence for silencing.

RNA-induced silencing complex, or RISC, is a multiprotein complex that incorporates one strand of a small interfering RNA (siRNA) or micro RNA (miRNA). RISC uses the siRNA or miRNA as a template for recognizing complementary mRNA. When it finds a complementary strand, it activates RNase and cleaves the RNA.

2021年12月22日 · Small interfering RNAs (siRNAs) and microRNAs (miRNAs) form RNA-induced silencing complex (RISC) together with a member of the Argonaute protein family to silence their complementary target mRNAs. In this review, Iwakawa and Tomari discuss the life and times of RISC—how RISC is formed, how it acts to regulate targets, and how it is degraded.

2022年1月6日 · To achieve these functions, small RNAs form RNA-induced silencing complex (RISC) together with a member of the Argonaute (AGO) protein family. RISC is directed by its bound small RNA to target complementary RNAs and represses their expression through mRNA cleavage, degradation, and/or translational repression.

The RNA-induced silencing complex is an extremely versatile basic and vital cellular regulatory machine of almost all the eukaryotic cells. This complex can be loaded with numerous naturally occurring guide RNA or in vitro man-made artificial siRNA or miRNA of any desired sequence and this sequence specific RNA can be adapted to serve various ...

2024年8月10日 · The RNA-induced silencing complex (RISC) is a multiprotein complex that plays a central role in RNA interference (RNAi) and post-transcriptional gene silencing mechanisms. RISC is responsible for recognizing small RNA molecules, such as microRNAs (miRNAs) and small interfering RNAs (siRNAs), and guiding them to target mRNAs for degradation or ...

2005年1月14日 · Recently, Grewal, Moazed and co-workers demonstrated the existence of an RNA-induced transcriptional silencing (RITS) complex that contains small repeat-associated RNAs, the Argonaute (Ago ...