SH-SY5Y is a human derived cell line used in scientific research. The original cell line, called SK-N-SH, from which it was subcloned was isolated from a bone marrow biopsy taken from a four-year-old female with neuroblastoma. SH-SY5Y cells are often used as in …

The SH-SY5Y cell line is a thrice cloned subline of the neuroblastoma cell line SK-N-SH (ATCC HTB-11), which was established in 1970 from a metastatic bone tumor from a 4-year-old cancer patient. Applications include use as a transfection host or in immunology, neuroscience, and toxicology research.

SH-SY5Y cells differentiate primarily to a cholinergic neuron phenotype in response to RA treatment, as evidenced by increased expression of choline acetyl transferase (ChAT) activity and vesicular monamine transporter (VMAT) expression [7, 15].

SH-SY5Y is a thrice-subcloned cell line derived from the SK-N-SH neuroblastoma cell line. It serves as a model for neurodegenerative disorders since the cells can be converted to various types of functional neurons by the addition of specific compounds.

In summary, SH-SY5Y cells can be cultivated at ideal conditions to optimize cell growth phases and cell densities and prevent excessive cell death when considering the culture conditions that were described in this study.

In this study, we present an optimized neuronal differentiation protocol for SH-SY5Y that requires only two work steps and 6 days. After differentiation, the cells present increased levels of ATP and plasma membrane activity but reduced expression of energetic stress response genes.

The SH-SY5Y human neuroblastoma cell line (ATCC® CRL-2266™) is one of the most used cell lines in neurosciences, either undifferentiated or differentiated into neuron-like cells.

Oct 7, 2024 · SH-SY5Y cells, a human-derived neuroblastoma cell line, have become pivotal in the study of neurological processes and drug discovery. These cells offer researchers a reliable and versatile model system for understanding complex neural mechanisms due to …

SH-SY5Y is a human-derived cell line that originated from subcloning of the SK-N-SH neuroblastoma cell line in 1970. The parent cell line, SK-N-SH, was developed from the bone marrow biopsy of a four-year-old female neuroblastoma patient.

In this context, the popular SH-SY5Y neuroblastoma cell line and its use in in vitro systems is described. Originally derived from a metastatic bone tumor biopsy, SH-SY5Y (ATCC (®) CRL-2266™) cells are a subline of the parental line SK-N-SH (ATCC (®) HTB-11™).