Intracellular recording or “sharp electrode recording” uses micropipettes with very small openings, usually a few hundred mega ohms in resistance. Dye is typically included in the pipette solution to allow the cell to be filled and the cellular morphology examined.

2022年8月4日 · This chapter explores extracellular (multi-cell recording and cell-attached patch) and intracellular recording techniques (inside-out patch, outside-out patch, sharp-electrode recordings, whole-cell patch).

2011年11月2日 · Intracellular recordings form a group of techniques used to measure with precision the voltage across, or electrical currents passing through, neuronal or other cellular membranes by inserting an electrode inside the neuron.

High-impedance, sharp intracellular electrodes were compared with whole cell patch electrodes by recording from single spinal neurons in immobilized frog tadpoles. A range of neuron properties were examined using sharp or patch test electrodes while making simultaneous recordings with a second contr …

In particular, the higher impedance of sharp electrodes results in large leak currents and precludes voltage-clamp recording. The patch-clamp method is the only way to capture the intracellular activity of single neurons with a high signal-to-noise ratio [ 1 ].

In this case, sharp electrode recordings may be more suitable. This type of recordings requires micropipettes with a pore that is much smaller than those used for whole-cell recordings, thereby preventing most of the ion exchange between intracellular space and the internal pipette solution.

Comprehensive review of basic membrane biophysics, intracellular recording approaches, and experimental instrumentation used to measure transmembrane electrical signals from single neurons.

Pipettes for patch-clamp recording are formed using only heat and gravity, while sharp electrodes for intracellular recording use a combination of heat, gravity, and a motor.

2020年10月12日 · We have developed a technique to record neuronal activity by using a coaxial guide tube and sharp electrode assembly, which allows researchers to repeatedly and reliably perform intracellular...

Current clamp and voltage clamp recordings can be implemented using either sharp microelectrode or patch clamp electrodes. In sharp microelectrode recordings, a glass capillary is heated and pulled at high velocity to produce a minute “sharp” tip.