2023年12月25日 · In solid-phase peptide synthesis (SPPS) one peptide end is attached to water-insoluble polymer and remains protected throughout the entire peptide formation, meaning both fewer steps and simplified purification, the reagents …

2007年12月13日 · This protocol for solid-phase peptide synthesis (SPPS) is based on the widely used Fmoc/tBu strategy, activation of the carboxyl groups by aminium-derived coupling reagents and use of...

An introductory guide to solid phase peptide synthesis. Resins, amino acid derivatives, coupling reagents, common problems and their solutions, peptide cleavage from resins, peptide purification and storage are some of the topics discussed.

Peptide synthesis is pretty robust and fool proof; however, there are a few things that can really mess up the reproducibility of these protocols. Chief amongst them is the quality of DMF. It is incredibly important to use good quality amine-free DMF.

The concept of solid-phase peptide synthesis (SPPS) is to retain chemistry that has been proven in solution but to add a covalent attachment step that links the nascent peptide chain to an insoluble polymeric support (resin).

2013年1月1日 · This introductory chapter will provide an overview of some of the most common methods in solid-phase peptide synthesis (SPPS) but will start with solution synthesis of peptides to introduce some concepts.

2023年6月5日 · Solid phase peptide synthesis (SPPS) is a technique for peptide synthesis. It can be defined as a process in which a peptide anchored by its C-terminus to an insoluble polymer is assembled by the successive addition of the protected amino acids constituting its sequence.

Overview of Solid Phase Peptide Synthesis (SPPS) General Solid Phase Peptide Synthesis Scheme The general process for synthesizing peptides on a resin starts by attaching the first amino acid, the C-terminal residue, to the resin.

Essential protocols for the synthesis of fully deprotected peptides are presented including resin handling, coupling, capping, Fmoc-deprotection, final cleavage and disulfide bridge formation. Index Entries: Solid phase peptide synthesis (SPPS); resin; Fmoc SPPS; coupling reagents; protecting groups; anchoring; side reaction. 1. Introduction.

Solid phase synthesis is a process by which chemical transformations can be carried out on solid support in order to prepare a wide range of synthetic compounds. This idea was first developed by Bruce Merrifield to synthesize polypeptides and earned him the Nobel Prize in 1984.