004586 B6.Cg-Tg(Vil1-cre)997Gum/J These Vil1-cre mice express Cre recombinase in villus and crypt epithelial cells of the small and large intestines and may be useful in studies of intestinal organogenesis.

020282 B6.Cg-Tg(Vil1-cre/ERT2)23Syr/J vil-Cre-ER T2 mice carry a transgene with tamoxifen inducible Cre recombinase under the control of the 9 kb mouse Vil1 , villin 1, promoter, and may be useful for generating specific targeted mutants for studies of the crypt-villus axis of the intestinal epithelium.

Villin-controlled Cre-expression leads to intestinal MMR-deficiency, similar to Lynch syndrome. 50% of tumors in the small intestine are malignant. A high proportion of carcinomas in VCMsh2 LoxP/null mice are resistant to cisplatin and FOLFOX

The villin-Cre and villin-Cre-ERT2 mice provide valuable tools for studies of cell lineage allocation and gene function in the developing and adult intestine. We generated two complementary systems for Cre-mediated recombination of target genes in the mouse digestive epithelium and tested them with a Cre-reporter mouse strain.

Atg7 flox/flox mice 30 were bred with mice expressing the Cre recombinase from the villin promoter (villin-Cre) 24 to generate mice with conditional deletion of Atg7 in the intestinal epithelium (Atg7 flox/flox villin-Cre).

2020年2月23日 · Villin, encoded by Vil1, is an actin-binding protein expressed by intestinal, hepatobiliary, and renal tubular epithelial cells. 1 Transgenic mice in which the Vil1 promoter drives Cre recombinase expression are widely used …

The Villin-Cre model, established by two different groups as an intestine-specific Cre expression system, is well characterized. A 9-kb mouse villin regulatory region was used to drive Cre recombinase expression in intestinal epithelial cells [ 20 ].

Villin-cre, R226R placentas were stained using the X-Gal staining protocol as described by Burn to determine the location of β-galactosidase activity in mouse placentas (8). The results are described in expression as seen in Figure 4.

033019 B6N.Cg-Tg(Vil1-cre)20Syr/J Mice harboring the vil-Cre transgene have the 9-kb mouse, Vil1 , villin 1, promoter directing expression of Cre recombinase to the intestinal epithelia and kidney, and may be useful for generating conditional …

Summary: We generated two complementary systems for Cre-mediated recombination of target genes in the mouse digestive epithelium and tested them with a Cre-reporter mouse strain. Cre was expressed under the control of a 9 kb regulatory region of the murine villin gene (vil-Cre).